가토골수에서 유래된 골모세포의 하악골 결손부 이식시 골형성에 미치는 효과
The Effect of Bone Marrow-Derived Osteoblasts on Mandibular Deffect in Rabbit
박영주, 남정훈, 김보균, 전민수, 정재안, 이정원, 안장훈, 강태인, 박미희, 임성철,
소속 상세정보
박영주 ( Park Young-Ju ) - 한림대학교 의과대학 강남성심병원 구강악안면외과학교실
남정훈 ( Nam Jeong-Hun ) - 한림대학교 의과대학 강남성심병원 구강악안면외과학교실
김보균 ( Kim Bo-Kyun ) - 한림대학교의료원 강남성심병원 구강악안면외과학교실
전민수 ( Jeon Min-Su ) - 한림대학교의료원 강남성심병원 구강악안면외과학교실
정재안 ( Chung Jae-An ) - 한림대학교 의과대학 강남성심병원 구강악안면외과학교실
이정원 ( Lee Jeong-Won ) - 한림대학교 의과대학 강남성심병원 구강악안면외과학교실
안장훈 ( Ahn Jang-Hoon ) - 한림대학교 의과대학 강남성심병원 구강악안면외과학교실
강태인 ( Gang Tae-In ) - 한림대학교 의과대학 강남성심병원 구강악안면외과학교실
박미희 ( Park Mi-Hee ) - 한림대학교 의과대학 강남성심병원 구강악안면외과학교실
임성철 ( Lim Sung-Chul ) - 조선대학교 의과대학 병리학교실
KMID : 0360120100320040306
Abstract
Purpose: The purpose of this experiment was to evaluate the clinical effect of cultured autoglogous osteoblasts as a way to treat the defect of mandible in rabbits.
Materials and Methods: Twelve rabbits were used to determine the rate of osteogenesis. The osteoblasts were obtained from the iliac crest of rabbits using aspiration. They were then cultured in Dulbecco’s Modified Eagles’s Medium (DMEM) with beta-glycerophosophatate, L-ascorbicacid, and dexamethasone to proliferate and differentiate osteoprogenitor cells. The expression of osteogenic markers were detected by reverse transcription-polymerase chain reaction (RT-PCR) and silver nitrate staining techniques. Five, 10- mm holes were placed in each rabbit mandible to simulate defective regions with the use of a low speed trephine bur. In the experimental group, the previously cited defects were grafted with both activated osteoblastic and autogenous bone. The control group, however, was only grafted with autogenous bone. Both groups were then analyzed at 2, 4, and 8-week intervals using bone histomorphometric analysis.
Results: According to histomorphologic analysis, the rates of new bone formation at the 2, 4, and 8-week intervals were 36%, 51%, and 23% for the control group, respectively; 52%, 39%, and 28%, for the experimental group, respectively. The experimental group showed higher rates of new bone formation compared to the control group at both the 2-week and 8-week interval.
Conclusion: Bone marrow-derived osteoblasts seems to be a promising bone graft material.
키워드
Osteoblasts; Autogenous bone; Histomorphometric analysis
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